Fast Garnet GBC Base is a synthetic aromatic amine dye intermediate broadly used in azo dye production, histological staining, enzyme detection methods, and laboratory research applications. It is used as a diazonium coupling block to give bright red to garnet colors when coupled with appropriate coupling agents. In biological and biochemical laboratories, it is commonly employed in histochemistry and enzyme localization techniques, particularly for detecting phosphatase enzyme activity in tissue sections. The compound is valued for its strong color development, reliable coupling performance, and compatibility with established staining protocols.
CAS NO.- 97-56-3
Synonyms- o-Aminoazotoluene, 2-Aminoazotoluene, Solvent Yellow 3
| Physical Properties | |
| Chemical formula | C14H15N3 |
| IUPAC Name | 2-methyl-4-[(2-methylphenyl)diazenyl]aniline |
| Molecular weight | 225.29 g/mol |
| Solubility | Ethanol: 10 mg/mL |
| pH | 1.4～2.8 |
| Storage | Store at Room Temperature |
| Chemical Properties | |
| Color | Red brown |
| Physical state (20 °C) | Solid |
| Melting Point | 100.0 °C to 104.0 °C |
| λmax | 491 nm |
| Appearance | Orange to red-brown or dark brown powder |
| Pictograms : |   |
| Hazard Statements : | H317: May cause an allergic skin reaction H350: May cause cancer |
| Precautionary statements : | P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection. P302 + P352 : IF ON SKIN: Wash with plenty of water. P308 + P313 : IF exposed or concerned: Get medical advice/ attention. |
Fast Garnet GBC Base is a synthetic aromatic amine dye intermediate broadly used in azo dye production, histological staining, enzyme detection methods, and laboratory research applications. It is used as a diazonium coupling block to give bright red to garnet colors when coupled with appropriate coupling agents. In biological and biochemical laboratories, it is commonly employed in histochemistry and enzyme localization techniques, particularly for detecting phosphatase enzyme activity in tissue sections. The compound is valued for its strong color development, reliable coupling performance, and compatibility with established staining protocols.
CAS NO.- 97-56-3
Synonyms- o-Aminoazotoluene, 2-Aminoazotoluene, Solvent Yellow 3
| Physical Properties | |
| Chemical formula | C14H15N3 |
| IUPAC Name | 2-methyl-4-[(2-methylphenyl)diazenyl]aniline |
| Molecular weight | 225.29 g/mol |
| Solubility | Ethanol: 10 mg/mL |
| pH | 1.4～2.8 |
| Storage | Store at Room Temperature |
| Chemical Properties | |
| Color | Red brown |
| Physical state (20 °C) | Solid |
| Melting Point | 100.0 °C to 104.0 °C |
| λmax | 491 nm |
| Appearance | Orange to red-brown or dark brown powder |
| Pictograms : | |
| Hazard Statements : | H317: May cause an allergic skin reaction H350: May cause cancer |
| Precautionary statements : | P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection. P302 + P352 : IF ON SKIN: Wash with plenty of water. P308 + P313 : IF exposed or concerned: Get medical advice/ attention. |
Fast Garnet GBC Base is utilized in histochemistry, immunohistochemistry, enzyme detection assays, pathology research, and microscopy laboratories. It is very useful for the visualization of alkaline phosphatase activity of tissues.
Fast Garnet GBC Base typically forms bright red to garnet-colored azo reaction products when coupled with suitable substrates during staining procedures.
It is used as a chromogenic reagent in the localization and staining of enzymes in histology laboratories to identify particular parts in the cells and tissues of organisms.
Fast Garnet GBC Base is generally insoluble or only slightly soluble in water. Its solubility depends on the specific chemical form, with the free base being poorly soluble in water but soluble in certain organic solvents. Its water-soluble salt forms are available for staining and diagnostic use and are generally made into a staining solution or solvent for laboratory applications.
Fast Garnet GBC Base produces a colored insoluble azo dye in response to enzyme-generated intermediates, which allows direct visualization of the enzyme activity in a microscope.
Fast Garnet GBC Base is a potential carcinogen and should be handled with caution. It is always advisable to wear appropriate protective clothing when handling this product and to avoid prolonged contact, ingestion, and exposure to dust.
