

New Methylene Blue N Zinc Chloride Double Salt is a cationic thiazine dye supplied as a zinc chloride double salt for enhanced stability and performance. It is widely used in biological staining, hematology, microbiology, and photodynamic research. This compound belongs to the phenothiazinium dye family (commonly classified under Basic Blue dyes). The dye is structurally related to methylene blue but offers distinct staining properties, particularly in reticulocyte identification and cytological applications. The zinc chloride complex improves dye consistency, shelf stability, and staining efficiency compared to the free base.

CAS No: 2516-05-04Â
Synonyms: Blue 24, Methylene Blue N.
| Physical Properties | |
| Chemical formula | C18H22Cl3N3SZn |
| IUPAC Name | zinc;ethyl-[7-(ethylamino)-2,8-dimethylphenothiazin-3-ylidene]azanium;trichloride |
| Molecular weight | 484.2 g/mol |
| Solubility | Soluble in water |
| Form | Powder |
| Storage | Store at room temperature |
| Chemical Properties | |
| Color | Dark brown to dark purple |
| Physical State | Powder |
| Melting Point | >240 °C |
| λmax | 628–635 nm |
| Pictograms : | ![]() |
| Hazard Statements : | H315: Causes skin irritation. H319: Causes serious eye irritation. H335: May cause respiratory irritation. |
| Precautionary statements : | P261: Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray. P264: Wash thoroughly after handling. P271: Use only outdoors or in a well-ventilated area. P273: Avoid release to the environment. P280: Wear protective gloves/protective clothing/eye protection/face protection. |
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New Methylene Blue N Zinc Chloride Double Salt is a cationic thiazine dye supplied as a zinc chloride double salt for enhanced stability and performance. It is widely used in biological staining, hematology, microbiology, and photodynamic research. This compound belongs to the phenothiazinium dye family (commonly classified under Basic Blue dyes). The dye is structurally related to methylene blue but offers distinct staining properties, particularly in reticulocyte identification and cytological applications. The zinc chloride complex improves dye consistency, shelf stability, and staining efficiency compared to the free base.

CAS No: 2516-05-04Â
Synonyms: Blue 24, Methylene Blue N.
| Physical Properties | |
| Chemical formula | C18H22Cl3N3SZn |
| IUPAC Name | zinc;ethyl-[7-(ethylamino)-2,8-dimethylphenothiazin-3-ylidene]azanium;trichloride |
| Molecular weight | 484.2 g/mol |
| Solubility | Soluble in water |
| Form | Powder |
| Storage | Store at room temperature |
| Chemical Properties | |
| Color | Dark brown to dark purple |
| Physical State | Powder |
| Melting Point | >240 °C |
| λmax | 628–635 nm |
| Pictograms : | ![]() |
| Hazard Statements : | H315: Causes skin irritation. H319: Causes serious eye irritation. H335: May cause respiratory irritation. |
| Precautionary statements : | P261: Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray. P264: Wash thoroughly after handling. P271: Use only outdoors or in a well-ventilated area. P273: Avoid release to the environment. P280: Wear protective gloves/protective clothing/eye protection/face protection. |
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New Methylene Blue N Zinc Chloride Double Salt is primarily used as a biological stain in microscopy, reticulocyte counting, and research studies. It helps to visualize cellular structures and RNA-containing components in laboratory samples.
Methylene blue and New Methylene Blue N Zinc Chloride Double Salt are different dyes with distinct chemical properties. Reticulocyte staining is generally done by using New Methylene Blue N Zinc Chloride Double Salt, which interacts with the RNA structures. On the other hand, Methylene blue is widely used as a biological stain and redox indicator.
New Methylene Blue N Zinc Chloride Double Salt interacts with RNA in young red blood cells. This reaction builds visible blue-stained networks that can be identified and counted as reticulocytes under the microscope.
New Methylene Blue N Zinc Chloride Double Salt is a synthetic dye used in laboratory and research applications.
Zinc chloride improves the performance and stability of the dye, which makes it more useful for staining applications.
New Methylene Blue N Zinc Chloride Double Salt should be stored as per safety information, usually in a tightly closed container protected from adverse environmental conditions to maintain quality and stability.