Methylene Blue Anhydrous is a synthetic heterocyclic compound widely recognized for its distinctive staining properties and broad research applications. It is a dark green crystalline powder with a metallic sheen in its dry form. When dissolved in water or alcohol, it produces a deep blue solution. It is a cationic (basic) dye, binding easily to negatively charged cellular components such as DNA and RNA, which makes it a useful stain for biological, microbiological, and histological studies.
Beyond laboratory staining, it has been investigated for its oxidation-reduction properties and has been proven to be useful in scientific research, analytical testing, aquaculture, and specialized medical applications. It has also been investigated for its antioxidant, antimicrobial, and neuroprotective potential in pharmaceutical research.
CAS No- 61-73-4
Synonyms: Basic Blue 9, Solvent Blue 8, Swiss Blue
| Physical Properties | |
| Chemical formula | C16H18ClN3S |
| IUPAC Name | [7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium chloride |
| Molecular weight | 319.9 g/mol |
| Solubility | Soluble in water and Chloroform |
| Insolubility | Ether |
| Density | 1.757 g/cm3 (22 °C) |
| Appearance | Dark green crystals or powder |
| Storage | Store at room temperature |
| Chemical Properties | |
| Color | Dark Green |
| Physical State | Solid |
| Melting Point | 180 – 190 °C |
| λmax | 664 nm |
| Odor | Odorless |
| Pictograms : |  |
| Hazard Statements : | H302: Harmful if swallowed |
| Precautionary statements : | P270: Do not eat, drink, or smoke when using this product P264: Wash hands thoroughly after handling P301+P312: IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell |
Methylene Blue Anhydrous is a synthetic heterocyclic compound widely recognized for its distinctive staining properties and broad research applications. It is a dark green crystalline powder with a metallic sheen in its dry form. When dissolved in water or alcohol, it produces a deep blue solution. It is a cationic (basic) dye, binding easily to negatively charged cellular components such as DNA and RNA, which makes it a useful stain for biological, microbiological, and histological studies.
Beyond laboratory staining, it has been investigated for its oxidation-reduction properties and has been proven to be useful in scientific research, analytical testing, aquaculture, and specialized medical applications. It has also been investigated for its antioxidant, antimicrobial, and neuroprotective potential in pharmaceutical research.
CAS No- 61-73-4
Synonyms: Basic Blue 9, Solvent Blue 8, Swiss Blue
| Physical Properties | |
| Chemical formula | C16H18ClN3S |
| IUPAC Name | [7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium chloride |
| Molecular weight | 319.9 g/mol |
| Solubility | Soluble in water and Chloroform |
| Insolubility | Ether |
| Density | 1.757 g/cm3 (22 °C) |
| Appearance | Dark green crystals or powder |
| Storage | Store at room temperature |
| Chemical Properties | |
| Color | Dark Green |
| Physical State | Solid |
| Melting Point | 180 – 190 °C |
| λmax | 664 nm |
| Odor | Odorless |
| Pictograms : | |
| Hazard Statements : | H302: Harmful if swallowed |
| Precautionary statements : | P270: Do not eat, drink, or smoke when using this product P264: Wash hands thoroughly after handling P301+P312: IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell |
Methylene Blue Anhydrous is mainly used as a biological stain, bacteriological stain, oxidation-reduction indicator, fluorochrome, and research reagent in life science, chemistry, and analytical laboratories.
Methylene Blue Anhydrous should be used with accepted laboratory safety information. Do not touch directly because it can stain skin or clothing and may cause eye or skin irritation. Appropriate personal protective equipment (PPE) such as gloves and safety glasses should be worn during handling.
Methylene Blue Anhydrous readily dissolves in water, forming its characteristic deep blue solution. This solution is commonly used in biological staining, analytical testing, and other research applications.
Methylene Blue Anhydrous is positively charged and strongly binds to negatively charged cellular components such as DNA and RNA. This produces good contrast, which can be seen under the microscope and analyzed in the lab.
