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Acridine Orange Hemi Zinc Chloride

MacsChem > Biological Stains > Acridine Orange Hemi Zinc Chloride

What is Acridine Orange Hemi Zinc Chloride?

Acridine Orange Hemi Zinc Chloride is the zinc chloride salt form of Acridine Orange, a cationic fluorescent dye belonging to the acridine family. It is widely used in biological and biochemical applications and exhibits metachromatic properties, enabling differentiation between DNA and RNA. Due to its strong fluorescence, it is extensively applied in cell biology, microbiology, and fluorescence microscopy.

chemical-structure-of-acridine-orange-hemi-zinc-chloride

CAS No.: 10127-02-3

Synonyms: Basic Orange 14; Acridine Orange ZnClâ‚‚ salt

Properties of Acridine Orange Hemi Zinc Chloride

Physical Properties
Chemical formula C34H40Cl4N6Zn
IUPAC Namedichlorozinc;bis(3-N,3-N,6-N,6-N-tetramethylacridine-3,6-diamine);dihydrochloride
Molecular weight369.96 g/mol
SolubilitySoluble in water and ethanol
Chemical Properties
ColourReddish-brown
StateSolid Powder
Melting point165° C
OdorOdorless

Application of Acridine Orange Hemi Zinc Chloride

  • Acridine orange hemi(zinc chloride) salt is widely used as a nucleic acid-selective fluorescent dye, interacting with DNA through intercalation and with RNA via electrostatic attractions.
  • It is particularly useful for staining nucleic acids in cells or gels, such as agarose and polyacrylamide gels, making it valuable in molecular biology applications.
  • Acridine orange hemizinc chloride is also employed in cell cycle determination due to its ability to permeate cells and selectively stain nucleic acids, supporting cytometry and related studies.
  • It is used in fluorescence microscopy to visualize DNA and RNA within cells, aiding in cellular and molecular imaging.
  • Beyond staining, it functions as an inhibitor of RNA polymerase, useful in studies of transcriptional regulation.
  • As an organometallic compound, it also has applications in thin film deposition and industrial chemistry, where it can act as a reagent or catalyst.
Pictograms :pictogram-health-hazards
Hazard Statements :

H341 Suspected of causing genetic defects.

Precautionary statements :

P201 Obtain special instructions before use.

P202 Do not handle until all safety precautions have been read and

understood.

P280 Wear protective gloves/ protective clothing/ eye protection/ face

protection.

P308 + P313 IF exposed or concerned: Get medical advice/ attention.

P405 Store locked up.

P501 Dispose of contents/ container to an approved waste disposal

plant.

What is Acridine Orange Hemi Zinc Chloride?

Acridine Orange Hemi Zinc Chloride is the zinc chloride salt form of Acridine Orange, a cationic fluorescent dye belonging to the acridine family. It is widely used in biological and biochemical applications and exhibits metachromatic properties, enabling differentiation between DNA and RNA. Due to its strong fluorescence, it is extensively applied in cell biology, microbiology, and fluorescence microscopy.

chemical-structure-of-acridine-orange-hemi-zinc-chloride

CAS No.: 10127-02-3

Synonyms: Basic Orange 14; Acridine Orange ZnClâ‚‚ salt

Properties of Acridine Orange Hemi Zinc Chloride

Physical Properties
Chemical formula C34H40Cl4N6Zn
IUPAC Namedichlorozinc;bis(3-N,3-N,6-N,6-N-tetramethylacridine-3,6-diamine);dihydrochloride
Molecular weight369.96 g/mol
SolubilitySoluble in water and ethanol
Chemical Properties
ColourReddish-brown
StateSolid Powder
Melting point165° C
OdorOdorless

Application of Acridine Orange Hemi Zinc Chloride

  • Acridine orange hemi(zinc chloride) salt is widely used as a nucleic acid-selective fluorescent dye, interacting with DNA through intercalation and with RNA via electrostatic attractions.
  • It is particularly useful for staining nucleic acids in cells or gels, such as agarose and polyacrylamide gels, making it valuable in molecular biology applications.
  • Acridine orange hemizinc chloride is also employed in cell cycle determination due to its ability to permeate cells and selectively stain nucleic acids, supporting cytometry and related studies.
  • It is used in fluorescence microscopy to visualize DNA and RNA within cells, aiding in cellular and molecular imaging.
  • Beyond staining, it functions as an inhibitor of RNA polymerase, useful in studies of transcriptional regulation.
  • As an organometallic compound, it also has applications in thin film deposition and industrial chemistry, where it can act as a reagent or catalyst.
Pictograms :pictogram-health-hazards
Hazard Statements :

H341 Suspected of causing genetic defects.

Precautionary statements :

P201 Obtain special instructions before use.

P202 Do not handle until all safety precautions have been read and

understood.

P280 Wear protective gloves/ protective clothing/ eye protection/ face

protection.

P308 + P313 IF exposed or concerned: Get medical advice/ attention.

P405 Store locked up.

P501 Dispose of contents/ container to an approved waste disposal

plant.

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