MacsChem

MAC BLUE – Methylene Blue Powder (100 gms) for Research Purpose

Ultra-High Purity Methylene Blue Powder for Research Purpose
cGMP Manufactured
Free from Metals, Organic Impurities and Solvents
Also available in Packing size-  50 gm, 500 gm

View Detailed External Lab Report

(Kindly email us at sales@macschem.us for bulk pricing)
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SKU: MAC001-1 Category:

Description

Ultra High Purity Methylene Blue

  • Minimum 99.5% assay
  • Less than 1 ppm of heavy metals like mercury, arsenic, cadmium, and lead
  • Free from organic impurities like Azure A, B, C and Thionin
  • No residual Organic Solvents

The product will be packed in food-grade HDPE Jars. This product is shipped in powder form and can be mixed with water for preparing a solution (0.1%, 1%, 2%). Each shipment will be provided with a Batch Certificate of Analysis. 

(This product contains 100 gms of Methylene Blue Powder)

Also available in the packing size of 500 gm. Kindly email us at sales@macschem.us for bulk pricing.

Resources on Methylene Blue

Methylene Blue & Brain Health: Benefits, Mechanisms, and Potential Uses

Biological Stains | Classification, Examples & Uses

Methylene Blue Dye | Chemistry, Uses & Side effects

Staining with Methylene Blue

Methylene Blue’s uses in Fish Aquaculture

Methylene Blue in the treatment of Alzheimer’s

Methylene Blue in Covid-19

Methylene Blue Against Cyanide Poisoning

Malaria Treatment with Methylene Blue

Methylene Blue for Methemoglobinemia

Methylene Blue Injection: Indications, Dosage & Brands

Methylene Blue for Cancer Therapy

Disclaimer-

We do not claim or recommend any specific application for Methylene Blue. Use in accordance with the laws and regulations of your country or state. Any terms mentioned are solely intended to describe the product’s quality, not its intended usage.

This product is not an API, Bulk drug, Pharmaceutical or Medicine.

We do not supply patent infringing specifications material to applicable territories.

Product Specifications

CriteriaSpecification / Result
Appearance and OdorDark green or blue crystals or crystalline powder having a bronze-like luster; odorless or practically so and stable in air. Its solutions in water and in alcohol are deep blue in color.
Relative SolubilitySlightly soluble in water and in alcohol.
Identification
Infrared (IR) AbsorptionThe IR spectrum is concordant with the reference spectrum of Methylene Blue.
Retention Time (RT)The RT of the major peak of the sample solution corresponds to that of the standard solution as obtained in Assay.
Assay by HPLC (on dried basis)NLT 99.0% to NMT 101.0%
Related Substances
Azure-B< 2.5%
Azure-A< 0.1%
Azure-C< 0.1%
Organic Impurities
Specified Organic Impurities< 0.1%
Unspecified Organic Impurities< 0.05%
Residue on IgnitionNMT 0.15%
Heavy Metals
Mercury (Hg)NMT 0.00005%
Cadmium (Cd)NMT 0.00005%
Arsenic (As)NMT 0.00005%
Lead (Pb)NMT 0.00005%
Other MetalsNMT 0.005% (in total)
Specific Test
Loss on DryingNLT 8.0% – NMT 15.0% of its weight

Applications

Methylene Blue is widely used across research, laboratory, and industrial settings. Key applications include:

  • Biological staining: A cationic dye that binds negatively charged cell components such as DNA and RNA, used to visualise cells under the microscope and to assess cell viability.
  • Redox indicator: Turns blue when oxidised and colourless when reduced, making it useful in analytical chemistry.
  • EMB agar: Used in Eosin Methylene Blue agar to inhibit Gram-positive bacteria, making it selective for Gram-negative organisms such as E. coli.
  • Aquaculture: Acts as an aquarium disinfectant, controls fungal growth on fish eggs, and is active against parasitic protozoa and fungal infections.
  • Disinfectant: Deactivates viruses and pathogens when activated by light (photodynamic action).
  • Water testing: Serves as an indirect indicator of dissolved oxygen content and helps detect biological contamination.
  • Dairy industry: Used in the methylene blue reduction test to assess the microbiological quality of raw and pasteurised milk.

Disclaimer: We do not claim or recommend any specific application for this product. It is supplied for research purposes only and is not an API, bulk drug, pharmaceutical or medicine.

Third Party Testing

Our Methylene Blue is independently analysed by an accredited third-party laboratory to verify identity, purity, and heavy-metal limits. This ensures the material you receive consistently meets the specifications listed above, with full transparency and traceability.

What the external report confirms:

  • Assay (purity) by HPLC
  • Heavy metals – Mercury, Cadmium, Arsenic, Lead
  • Related substances (Azure A, B, C)
  • Identity confirmation by IR spectrum

A Batch Certificate of Analysis is supplied with every shipment. You can also view a sample of our detailed external lab report below.


View Detailed External Lab Report →

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