New Methylene blue is an organic compound that belongs to the thiazine class of heterocycles. It is classified as an azine dye, with a cationic chromophore and an often unspecified anion. Further appearing as a dark-green powder, it is highly soluble in water and ethanol, producing a blue-green solution. This dye has a strong affinity for acidic cellular components and is commonly used in various staining procedures. Its structure contains nitrogen and sulfur atoms, which contribute to its ability to interact with biological materials. New Methylene Blue is also stable under normal conditions, making it suitable for various scientific applications.
CAS No.: 1934-16-3
Synonyms: Basic Blue 24; Methylene Blue N; C.I. Basic Blue 24; 5GCZ112BCN; NSC-9605; 3,7-Bis(ethylamino)-2,8- dimethylphenothiazin-5 -ium chloride; Phenothiazin-5-ium, 3,7-bis (ethylamino)-2,8-dimethyl-,chloride
| Physical Properties | |
| Chemical formula | C18H22ClN3S |
| IUPAC Name | ethyl-[7-(ethylamino)-2,8-dimethylphenothiazin-3-ylidene]azanium;chloride |
| Molecular weight | 347.9 g/mol |
| Solubility | Soluble in Water & Ethanol |
| Density | 1.0 g/cm³ |
| Chemical Properties | |
| Colour | Dark Green |
| State | Crystalline Solid Powder |
| Melting point | 190 °C |
| Vapor pressure | < 0.01 hPa (20 °C) |
| pH | 2.5 – 3.5 |
| Pictograms : |   |
| Hazard Statements : | H316: Causes mild skin irritation |
| Precautionary statements : | P260: Do not breathe dust/ fume/ gas/ mist/ vapors/ spray. P280: Wear protective gloves/ eye protection/ face protection. P305+351+338: If in eyes: Rinse cautiously with water for several minutes. Remove contact lenses, if present, and easy to do. Continue rinsing. |
New Methylene blue is an organic compound that belongs to the thiazine class of heterocycles. It is classified as an azine dye, with a cationic chromophore and an often unspecified anion. Further appearing as a dark-green powder, it is highly soluble in water and ethanol, producing a blue-green solution. This dye has a strong affinity for acidic cellular components and is commonly used in various staining procedures. Its structure contains nitrogen and sulfur atoms, which contribute to its ability to interact with biological materials. New Methylene Blue is also stable under normal conditions, making it suitable for various scientific applications.
CAS No.: 1934-16-3
Synonyms: Basic Blue 24; Methylene Blue N; C.I. Basic Blue 24; 5GCZ112BCN; NSC-9605; 3,7-Bis(ethylamino)-2,8- dimethylphenothiazin-5 -ium chloride; Phenothiazin-5-ium, 3,7-bis (ethylamino)-2,8-dimethyl-,chloride
| Physical Properties | |
| Chemical formula | C18H22ClN3S |
| IUPAC Name | ethyl-[7-(ethylamino)-2,8-dimethylphenothiazin-3-ylidene]azanium;chloride |
| Molecular weight | 347.9 g/mol |
| Solubility | Soluble in Water & Ethanol |
| Density | 1.0 g/cm³ |
| Chemical Properties | |
| Colour | Dark Green |
| State | Crystalline Solid Powder |
| Melting point | 190 °C |
| Vapor pressure | < 0.01 hPa (20 °C) |
| pH | 2.5 – 3.5 |
| Pictograms : | |
| Hazard Statements : | H316: Causes mild skin irritation |
| Precautionary statements : | P260: Do not breathe dust/ fume/ gas/ mist/ vapors/ spray. P280: Wear protective gloves/ eye protection/ face protection. P305+351+338: If in eyes: Rinse cautiously with water for several minutes. Remove contact lenses, if present, and easy to do. Continue rinsing. |
New Methylene Blue stain helps identify immature red blood cells and reticulocytes in blood smears.
The principle of the New Methylene Blue test is based on its ability to stain RNA and organelles in immature red blood cells (reticulocytes). The dye binds to the reticulocyte’s RNA, forming a precipitate that makes these cells visible under a microscope.
The major contrast between methylene blue and new methylene blue is their staining properties. Methylene blue stains a broader range of cells, while new methylene blue is a supravital stain specifically used for reticulocytes.
A New Methylene Blue solution can be prepared by, dissolving the appropriate amount of New Methylene Blue powder in distilled water. For a typical solution, use 0.1% to 1% concentration. Mix thoroughly until fully dissolved.
New Methylene Blue blood stain is a staining solution utilized to highlight immature red blood cells and other cell types in blood samples. It helps in the identification and differentiation of cellular components under a microscope.
