Neutral Red known as toluylene red, is a eurhodin often used in histology and microbiology. It stains lysosomes and other cellular components and can also be employed as a vital stain to study live cells. It functions as a pH indicator, transiting from red to yellow between pH 6.8 and 8.0, and is available as a chloride salt for various staining methods.
CAS No.: 553-24-2
Synonyms: Toluylene Red; Basic Red 5; 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride; C.I. Basic Red 5; Neutral Red chloride; N2,N2,7-Trimethylphenazine-2,8-diamine hydrochloride; Neutral Red W.
| Physical Properties | |
| Chemical formula | C15H17ClN4 |
| IUPAC Name | 8-N,8-N,3-trimethylphenazine-2,8-diamine;hydrochloride |
| Molecular weight | 288.77 g/mol |
| Solubility | Water & Ethanol |
| Density | 1.1590 g/cm³ |
| Chemical Properties | |
| Color | Dark Green |
| State | Powder |
| Melting point | 290°C |
| λmax | 530-540 nm |
| pH range | 6.8 (red) – 8 (yellow) |
| pH | 3 at 20 °C |
| Pictograms : |
|
| Hazard Statements : | H315: Causes skin irritation |
| Precautionary statements : | P203: Obtain, read, and follow all safety instructions before use. |
Neutral Red known as toluylene red, is a eurhodin often used in histology and microbiology. It stains lysosomes and other cellular components and can also be employed as a vital stain to study live cells. It functions as a pH indicator, transiting from red to yellow between pH 6.8 and 8.0, and is available as a chloride salt for various staining methods.
CAS No.: 553-24-2
Synonyms: Toluylene Red; Basic Red 5; 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride; C.I. Basic Red 5; Neutral Red chloride; N2,N2,7-Trimethylphenazine-2,8-diamine hydrochloride; Neutral Red W.
| Physical Properties | |
| Chemical formula | C15H17ClN4 |
| IUPAC Name | 8-N,8-N,3-trimethylphenazine-2,8-diamine;hydrochloride |
| Molecular weight | 288.77 g/mol |
| Solubility | Water & Ethanol |
| Density | 1.1590 g/cm³ |
| Chemical Properties | |
| Color | Dark Green |
| State | Powder |
| Melting point | 290°C |
| λmax | 530-540 nm |
| pH range | 6.8 (red) – 8 (yellow) |
| pH | 3 at 20 °C |
| Pictograms : |
|
| Hazard Statements : | H315: Causes skin irritation |
| Precautionary statements : | P203: Obtain, read, and follow all safety instructions before use. |
Neutral Red is a positively charged (cationic) dye.
The principle of Neutral Red is that live cells incorporate the dye into their lysosomes, staining them red. It is used to assess cell viability, as dead or damaged cells lose the ability to uptake the dye.
Neutral Red is a weak cationic dye.
Neutral Red is used for staining cells and tissues in histology and microbiology. It helps identify lysosomes, differentiate bacteria, and assess cell viability.
Safranin is more commonly used in Gram; however, Neutral red can be used in Gram staining as a counterstain.Â
