Ponceau S is a light red diazo dye available as a sodium salt. It is used to prepare a stain for the rapid detection of protein bands on nitrocellulose and PVDF membranes during Western blotting. As the stain is reversible, it can be easily removed after staining, allowing the membrane to be used for antibody detection without affecting later steps in the experiment. Its staining solution contains 0.5% Ponceau S in 1% glacial acetic acid. Once stained, the membrane develops red bands, which are visible against a clear background, and it can be easily confirmed that proteins have transferred to the membrane.
In addition to Western blotting, Ponceau S stain is used to stain proteins on gel electropherograms and cellulose acetate membranes. It is also used in botanical research to stain sieve plate elements. Because it provides fast, inexpensive, and reversible protein staining, it has become a routine quality control step in many molecular biology laboratories.
CAS No.: 6226-79-5Â
Synonyms: Lichun red; ACID RED 112; Ponceau Red S.
| Physical Properties | |
| Chemical formula | C22H16N4O13S4.4Na |
| IUPAC Name | tetrasodium 3-hydroxy-4-{2-sulfonato-4-[(4-sulfonatophenyl)diazenyl]phenyl}diazenyl]naphthalene-2,7-disulfonate |
| Molecular weight | 760.57 g/mol |
| Solubility | Soluble in water |
| Form | Powder |
| Storage | Store at Room Temperature |
| Chemical Properties | |
| Color | Dark red crystalline |
| Ph value | 9 |
| Odor | Characteristic odor |
| Pictograms : |  |
| Hazard Statements : | H315: Causes skin irritation H319: Causes serious eye irritation |
| Precautionary statements : | P280: Wear protective gloves/protective clothing/eye protection/face protection. P302+P352: IF ON SKIN: wash with plenty of water. P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do – continue rinsing. P332+P313: IF SKIN irritation occurs: Get medical advice/attention. P337+P313: IF eye irritation persists: Get medical advice/attention |
Ponceau S is a light red diazo dye available as a sodium salt. It is used to prepare a stain for the rapid detection of protein bands on nitrocellulose and PVDF membranes during Western blotting. As the stain is reversible, it can be easily removed after staining, allowing the membrane to be used for antibody detection without affecting later steps in the experiment. Its staining solution contains 0.5% Ponceau S in 1% glacial acetic acid. Once stained, the membrane develops red bands, which are visible against a clear background, and it can be easily confirmed that proteins have transferred to the membrane.
In addition to Western blotting, Ponceau S stain is used to stain proteins on gel electropherograms and cellulose acetate membranes. It is also used in botanical research to stain sieve plate elements. Because it provides fast, inexpensive, and reversible protein staining, it has become a routine quality control step in many molecular biology laboratories.
CAS No.: 6226-79-5Â
Synonyms: Lichun red; ACID RED 112; Ponceau Red S.
| Physical Properties | |
| Chemical formula | C22H16N4O13S4.4Na |
| IUPAC Name | tetrasodium 3-hydroxy-4-{2-sulfonato-4-[(4-sulfonatophenyl)diazenyl]phenyl}diazenyl]naphthalene-2,7-disulfonate |
| Molecular weight | 760.57 g/mol |
| Solubility | Soluble in water |
| Form | Powder |
| Storage | Store at Room Temperature |
| Chemical Properties | |
| Color | Dark red crystalline |
| Ph value | 9 |
| Odor | Characteristic odor |
| Pictograms : | |
| Hazard Statements : | H315: Causes skin irritation H319: Causes serious eye irritation |
| Precautionary statements : | P280: Wear protective gloves/protective clothing/eye protection/face protection. P302+P352: IF ON SKIN: wash with plenty of water. P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do – continue rinsing. P332+P313: IF SKIN irritation occurs: Get medical advice/attention. P337+P313: IF eye irritation persists: Get medical advice/attention |
Ponceau S is primarily used to stain proteins on nitrocellulose and PVDF membranes during Western blotting. It is useful to confirm the transfer efficiency of protein prior to the detection of antibody and for staining protein patterns on gel electropherograms and cellulose acetate membranes.
The Ponceau S can be used a number of times, depending on its condition and laboratory practice. After the staining solution has lost its effectiveness or become contaminated, it needs to be replaced for consistent staining results.
Ponceau S stains protein bands within a few minutes. The exact staining time may vary depending on the membrane and the amount of protein transferred.
Ponceau S staining involves placing the membrane in the staining solution until the protein bands appear. Rinse the membrane with water after staining to remove excess dye before continuing with immunodetection.
Ponceau S is a reversible stain. The red protein bands can be washed away with water, allowing the same membrane to be used for antibody-based detection.
