Methylene Violet is a synthetic cationic thiazine dye widely used as a biological staining reagent. It exhibits a strong affinity for negatively charged cellular components, allowing clear visualization of cells and tissues during microscopic observations. Its high staining efficiency, aqueous solubility, and consistent staining performance make it suitable for a variety of laboratory staining procedures. It is mainly used in histology, hematology, cytology, and pathology. It is commonly incorporated into multichromatic staining formulations, including MacNeal’s tetrachrome stain for blood, and is widely utilized in clinical diagnostics, biomedical research, and educational laboratories.
CAS No: 2516-05-04Â
Synonyms: Methylene violet Bernthsen.
| Physical Properties | |
| Chemical formula | C14H12N2OS |
| IUPAC Name | 7-(dimethylamino)phenothiazin-3-one |
| Molecular weight | 256.32 g/mol |
| Solubility | Insoluble in water |
| Flash point | 213.6 °C |
| Form | Powder |
| Storage | Store in a cool, dry place. |
| Chemical Properties | |
| Color | Dark green |
| Physical State | Solid |
| Melting Point | 216 °C |
| Vapor Pressure | 1.38E-07mmHg at 25°C |
| Pictograms : | N/A |
| Hazard Statements : | N/A |
| Precautionary statements : | N/A |
Methylene Violet is a synthetic cationic thiazine dye widely used as a biological staining reagent. It exhibits a strong affinity for negatively charged cellular components, allowing clear visualization of cells and tissues during microscopic observations. Its high staining efficiency, aqueous solubility, and consistent staining performance make it suitable for a variety of laboratory staining procedures. It is mainly used in histology, hematology, cytology, and pathology. It is commonly incorporated into multichromatic staining formulations, including MacNeal’s tetrachrome stain for blood, and is widely utilized in clinical diagnostics, biomedical research, and educational laboratories.
CAS No: 2516-05-04Â
Synonyms: Methylene violet Bernthsen.
| Physical Properties | |
| Chemical formula | C14H12N2OS |
| IUPAC Name | 7-(dimethylamino)phenothiazin-3-one |
| Molecular weight | 256.32 g/mol |
| Solubility | Insoluble in water |
| Flash point | 213.6 °C |
| Form | Powder |
| Storage | Store in a cool, dry place. |
| Chemical Properties | |
| Color | Dark green |
| Physical State | Solid |
| Melting Point | 216 °C |
| Vapor Pressure | 1.38E-07mmHg at 25°C |
| Pictograms : | N/A |
| Hazard Statements : | N/A |
| Precautionary statements : | N/A |
Methylene Violet is mainly used as a certified stain and dye in cell biology, hematology, and histology. It aids researchers in visualizing cellular structures under a microscope and supports analytical research applications.
Methylene Violet functions as a cationic dye by binding to negatively charged cellular components. This selective binding enhances the contrast of cells and tissues, making microscopic structures easier to distinguish and analyze.
Methylene Violet is used as a standard for consistent testing and formulation in analytical research. It is also used for quality control studies and for method validation.
Methylene Violet and Crystal Violet have different properties and are different dyes. They are used in staining techniques but differ in chemical behavior and in the specific laboratory uses.
Methylene Violet is used in pharmaceutical research for analytical testing, impurity studies, and quality control applications.
