
Martius Yellow is an azo dye compound prepared by the nitration of naphthol. It is widely used as a biological stain and analytical reagent. It is an erythrocyte dye and is incorporated in the MSB (Martius Scarlet Blue) method, introduced by Lendrum in 1962, for the selective staining of fibrin. It is often mixed with other low molecular-weight dyes to enhance stain intensity.

CAS No.: 605-69-6
Synonyms: 2,4-Dinitronaphthol; Martinsgelb; Acid yellow 24; Golden Yellow; Saffron Yellow; Manchester Yellow; Zlut naftolova; Martinsgelb
| Physical Properties | |
| Chemical formula | C10H6N2O5 |
| IUPAC Name | 2,4-Dinitronaphthalen-1-ol |
| Molar Mass | 234.167 g·mol−1 |
| Solubility | Soluble in water and organic solvents |
| Chemical Properties | |
| Color | Yellow – orange |
| State | Powder |
| Odor | Characteristic |
| Storage | Store at Room Temperature |
Histology & Microscopy
Analytical & Laboratory Use
Dye Industry
| Pictograms : |
|
| Hazard Statements : | H315 Causes skin irritation |
| Precautionary statements : | P280 Wear protective gloves/protective clothing/eye protection/face protection |
Martius Yellow is an azo dye compound prepared by the nitration of naphthol. It is widely used as a biological stain and analytical reagent. It is an erythrocyte dye and is incorporated in the MSB (Martius Scarlet Blue) method, introduced by Lendrum in 1962, for the selective staining of fibrin. It is often mixed with other low molecular-weight dyes to enhance stain intensity.

CAS No.: 605-69-6
Synonyms: 2,4-Dinitronaphthol; Martinsgelb; Acid yellow 24; Golden Yellow; Saffron Yellow; Manchester Yellow; Zlut naftolova; Martinsgelb
| Physical Properties | |
| Chemical formula | C10H6N2O5 |
| IUPAC Name | 2,4-Dinitronaphthalen-1-ol |
| Molar Mass | 234.167 g·mol−1 |
| Solubility | Soluble in water and organic solvents |
| Chemical Properties | |
| Color | Yellow – orange |
| State | Powder |
| Odor | Characteristic |
| Storage | Store at Room Temperature |
Histology & Microscopy
Analytical & Laboratory Use
Dye Industry
| Pictograms : |
|
| Hazard Statements : | H315 Causes skin irritation |
| Precautionary statements : | P280 Wear protective gloves/protective clothing/eye protection/face protection |
Martius Yellow (chemically 2,4-dinitro-1-naphthol) is synthesized by the nitration of 1-naphthol. During the reaction, nitro (–NO₂) groups are introduced into the aromatic naphthol ring, and controlled nitration leads to substitution at the 2 and 4 positions relative to the hydroxyl (–OH) group, producing 2,4-dinitro-1-naphthol (Martius Yellow).
Martius Yellow is a synthetic azo dye used to visualize erythrocytes and fibrin in connective tissue, and is also used as a reagent to detect specific metal ions and to measure pH.