Light Green SF Yellowish (Acid Green 5) is a synthetic anionic triarylmethane dye commonly used as a histological counterstain in microscopy and tissue staining. It is often available as the disodium salt and gives a distinct blue-green color, which improves the contrast of biological tissues and cells. It has excellent staining performance, but its relatively low color stability has led to the use of Fast Green FCF as a more durable alternative in some laboratory applications.Â
CAS No.: 5141-20-8
Synonyms: Light Green SF Yellowish, Acid Green 5, C.I. Acid Green 5, A F Green No.2, D&C Green No. 2
| Physical Properties | |
| Chemical formula | C37H34N2Na2O9S3 |
| IUPAC Name | disodium;3-[[N-ethyl-4-[[4-[ethyl-[(3-sulfonatophenyl)methyl]azaniumylidene]cyclohexa-2,5-dien-1-ylidene]-(4-sulfonatophenyl)methyl]anilino]methyl]benzenesulfonate |
| Molecular weight | 792.9 g/mol |
| Solubility | Soluble in water |
| Storage | Store at Room Temperature |
| Chemical Properties | |
| Color | Dark violet |
| Physical State | Solid Powder, Crystals |
| Melting Point | 288 °C |
| λmax | 630nm, 430nm |
| Pictograms : |   |
| Hazard Statements : | H302: Harmful if swallowed. H361: Suspected of damaging fertility or the unborn child. |
| Precautionary statements : | P280: Wear protective gloves/protective clothing/eye protection/face protection. P301+P312: IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. P330: Rinse mouth. P308+P313: IF exposed or concerned: Get medical advice/attention. |
Light Green SF Yellowish (Acid Green 5) is a synthetic anionic triarylmethane dye commonly used as a histological counterstain in microscopy and tissue staining. It is often available as the disodium salt and gives a distinct blue-green color, which improves the contrast of biological tissues and cells. It has excellent staining performance, but its relatively low color stability has led to the use of Fast Green FCF as a more durable alternative in some laboratory applications.Â
CAS No.: 5141-20-8
Synonyms: Light Green SF Yellowish, Acid Green 5, C.I. Acid Green 5, A F Green No.2, D&C Green No. 2
| Physical Properties | |
| Chemical formula | C37H34N2Na2O9S3 |
| IUPAC Name | disodium;3-[[N-ethyl-4-[[4-[ethyl-[(3-sulfonatophenyl)methyl]azaniumylidene]cyclohexa-2,5-dien-1-ylidene]-(4-sulfonatophenyl)methyl]anilino]methyl]benzenesulfonate |
| Molecular weight | 792.9 g/mol |
| Solubility | Soluble in water |
| Storage | Store at Room Temperature |
| Chemical Properties | |
| Color | Dark violet |
| Physical State | Solid Powder, Crystals |
| Melting Point | 288 °C |
| λmax | 630nm, 430nm |
| Pictograms : | |
| Hazard Statements : | H302: Harmful if swallowed. H361: Suspected of damaging fertility or the unborn child. |
| Precautionary statements : | P280: Wear protective gloves/protective clothing/eye protection/face protection. P301+P312: IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. P330: Rinse mouth. P308+P313: IF exposed or concerned: Get medical advice/attention. |
Light Green SF selectively stains collagen fibers. It is often used in Masson’s Trichrome staining to differentiate connective tissue.
Light Green SF staining solution is prepared by dissolving the dye in an appropriate solvent according to the recommended laboratory staining protocol for the intended application.
Light Green SF is used to enhance the visualization of collagen fibers, connective tissues, and cellular components in histological and cytological specimens.
Light Green SF is mainly used for staining collagen fibers and gives a blue-green color to the cytoplasm of some epithelial cells in histological and cytological staining techniques.
