Janus Green B is an organic chloride salt that belongs to the phenazine class of basic dyes. It occurs as a dark green to black powder and serves as a vital stain and oxidation-reduction indicator, exhibiting a blue color in its oxidized form and a pink color when reduced. It is an important stain used to selectively stain living cells, especially mitochondria, enabling Janus Green B mitochondrial staining for assessing cellular respiratory activity.Â
CAS No.: 2869-83-2
Synonyms: Janus Green V, Diazin green SÂ
| Physical Properties | |
| Chemical formula | C30H31ClN6 |
| IUPAC Name | 8-[[4-(dimethylamino)phenyl]diazenyl]-N,N-diethyl-10-phenylphenazin-10-ium-2-amine chloride |
| Molecular weight | 511.1 g/mol |
| Solubility | Soluble in water |
| Appearance | Dark green to Dark blue to Black powder to crystal |
| Storage | Store at Room Temperature |
| Chemical Properties | |
| Color | Bluish black to black |
| Physical State | Solid Powder |
| Melting Point | 160 °C |
| λmax | 395 nm, 660 nm |
| Pictograms : | No information available |
| Hazard Statements : | No information available |
| Precautionary statements : | No information available |
Janus Green B is an organic chloride salt that belongs to the phenazine class of basic dyes. It occurs as a dark green to black powder and serves as a vital stain and oxidation-reduction indicator, exhibiting a blue color in its oxidized form and a pink color when reduced. It is an important stain used to selectively stain living cells, especially mitochondria, enabling Janus Green B mitochondrial staining for assessing cellular respiratory activity.Â
CAS No.: 2869-83-2
Synonyms: Janus Green V, Diazin green SÂ
| Physical Properties | |
| Chemical formula | C30H31ClN6 |
| IUPAC Name | 8-[[4-(dimethylamino)phenyl]diazenyl]-N,N-diethyl-10-phenylphenazin-10-ium-2-amine chloride |
| Molecular weight | 511.1 g/mol |
| Solubility | Soluble in water |
| Appearance | Dark green to Dark blue to Black powder to crystal |
| Storage | Store at Room Temperature |
| Chemical Properties | |
| Color | Bluish black to black |
| Physical State | Solid Powder |
| Melting Point | 160 °C |
| λmax | 395 nm, 660 nm |
| Pictograms : | No information available |
| Hazard Statements : | No information available |
| Precautionary statements : | No information available |
Janus Green B solution is a ready-to-use staining reagent containing Janus Green B dye, used for supravital staining of living cells, especially for the visualization of mitochondria during microscopic observation.
Janus Green B solution is prepared by dissolving the appropriate quantity of the dye in a suitable solvent or buffer according to the recommended laboratory staining protocol. The preparation method and concentration may vary depending on the intended application.
Janus Green B is an oxidation-reduction indicator. In the presence of oxygen, the dye is oxidized and appears in blue, while under reduced conditions it changes to pink. This reversible property allows selective visualization of mitochondria and measurement of cellular respiratory activity.
Janus Green B stain is used for supravital staining of mitochondria, staining of nucleic acids and chromosomes, determination of cell density, and microscopic studies of various biological specimens, including fungi, yeast cells, nervous tissue, sperm cells, and tissue culture monolayers.
