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2,6-Dichloroindophenol Sodium Salt

MacsChem > Fine chemicals & Intermediates > 2,6-Dichloroindophenol Sodium Salt

What is 2,6-Dichloroindophenol Sodium Salt?

2,6-Dichloroindophenol Sodium Salt, also known as 2,6-Dichlorophenolindophenol Sodium Salt Dihydrate (DCPIP, DCIP, or DPIP), is a synthetic indophenol dye widely used as a redox indicator and analytical reagent. It is best known for the determination of vitamin C (ascorbic acid), where it changes from its blue oxidized form to a colorless reduced form during the reaction. In its oxidized state, it shows a maximum absorption of about 600 nm, making it valuable for spectrophotometric and oxidation-reduction analyses.

Structure of 2,6-Dichloroindophenol Sodium Salt

CAS No.: 620-45-1

Synonyms: 2,6-Dichlorobenzenone-indophenolsodium salt, Tillman’s reagent, 2,6-Dichlorophenol Indophenol Sodium Salt 

Properties of 2,6-Dichloroindophenol Sodium Salt

Physical Properties
Chemical formulaC12H6Cl2NNaO2
IUPAC Namesodium 4-[(3,5-dichloro-4-oxocyclohexa-2,5-dien-1-ylidene)amino]phenolate
Molecular weight290.07 g/mol
SolubilitySoluble in water
AppearanceDark green to Dark purple powder
StorageStore at room temperature
Chemical Properties
ColorDark Green
Physical State at 20 °CSolid
Melting Point>300 °C
OdorOdorless

Uses of 2,6-Dichloroindophenol Sodium Salt

  • 2,6-Dichloroindophenol sodium salt is commonly employed as an analytical reagent in the quantitative analysis of vitamin C (ascorbic acid). In the reaction, it is reduced from the blue to the colorless form.
  • It serves as a reliable oxidation-reduction (redox) dye in analytical chemistry for monitoring electron transfer reactions.
  • The dye is a Hill reagent and accepts electrons from the photosynthetic electron transport chain. By analyzing the photosynthetic activity spectrophotometrically, the rate of photosynthesis can be measured.
  • It has a high affinity for electrons and is therefore used as an artificial electron acceptor in biochemical and enzymatic studies.
  • It can be used in colorimetric and spectrophotometric assays for analytical measurements by its characteristic absorbance at ~600 nm.
  • It is used for staining glutathione reductase electrophoresis gels for visualization of the enzyme in biochemical research.
  • The compound is widely employed in research laboratories for oxidation-reduction studies, enzyme assays, and other analytical investigations.
Pictograms :Warning pictogram
Hazard Statements :

H315: Causes skin irritation. 

H319: Causes serious eye irritation. 

H335: May cause respiratory irritation.

Precautionary statements :

P261: Avoid breathing dust/fume/gas/mist/vapours/spray. 

P304+P340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. 

P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

 

 

What is 2,6-Dichloroindophenol Sodium Salt?

2,6-Dichloroindophenol Sodium Salt, also known as 2,6-Dichlorophenolindophenol Sodium Salt Dihydrate (DCPIP, DCIP, or DPIP), is a synthetic indophenol dye widely used as a redox indicator and analytical reagent. It is best known for the determination of vitamin C (ascorbic acid), where it changes from its blue oxidized form to a colorless reduced form during the reaction. In its oxidized state, it shows a maximum absorption of about 600 nm, making it valuable for spectrophotometric and oxidation-reduction analyses.

Structure of 2,6-Dichloroindophenol Sodium Salt

CAS No.: 620-45-1

Synonyms: 2,6-Dichlorobenzenone-indophenolsodium salt, Tillman’s reagent, 2,6-Dichlorophenol Indophenol Sodium Salt 

Properties of 2,6-Dichloroindophenol Sodium Salt

Physical Properties
Chemical formulaC12H6Cl2NNaO2
IUPAC Namesodium 4-[(3,5-dichloro-4-oxocyclohexa-2,5-dien-1-ylidene)amino]phenolate
Molecular weight290.07 g/mol
SolubilitySoluble in water
AppearanceDark green to Dark purple powder
StorageStore at room temperature
Chemical Properties
ColorDark Green
Physical State at 20 °CSolid
Melting Point>300 °C
OdorOdorless

Uses of 2,6-Dichloroindophenol Sodium Salt

  • 2,6-Dichloroindophenol sodium salt is commonly employed as an analytical reagent in the quantitative analysis of vitamin C (ascorbic acid). In the reaction, it is reduced from the blue to the colorless form.
  • It serves as a reliable oxidation-reduction (redox) dye in analytical chemistry for monitoring electron transfer reactions.
  • The dye is a Hill reagent and accepts electrons from the photosynthetic electron transport chain. By analyzing the photosynthetic activity spectrophotometrically, the rate of photosynthesis can be measured.
  • It has a high affinity for electrons and is therefore used as an artificial electron acceptor in biochemical and enzymatic studies.
  • It can be used in colorimetric and spectrophotometric assays for analytical measurements by its characteristic absorbance at ~600 nm.
  • It is used for staining glutathione reductase electrophoresis gels for visualization of the enzyme in biochemical research.
  • The compound is widely employed in research laboratories for oxidation-reduction studies, enzyme assays, and other analytical investigations.
Pictograms :Warning pictogram
Hazard Statements :

H315: Causes skin irritation. 

H319: Causes serious eye irritation. 

H335: May cause respiratory irritation.

Precautionary statements :

P261: Avoid breathing dust/fume/gas/mist/vapours/spray. 

P304+P340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. 

P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

 

 

FAQ's

2,6-Dichloroindophenol Sodium Salt is mainly used for vitamin C determination, redox reactions, photosynthesis studies, spectrophotometric analysis, electron transfer experiments, and electrophoresis staining.

2,6-Dichloroindophenol Sodium Salt (DCPIP) functions as a redox dye. In its oxidized state it is blue, and when it accepts electrons during a reduction reaction, it becomes colorless.

2,6-Dichloroindophenol Sodium Salt should be handled using appropriate laboratory safety practices such as wearing suitable personal protective equipment, avoiding inhalation or ingestion, and follow the safety instructions provided in the product Safety Data Information.

2,6-Dichloroindophenol Sodium Salt acts as an artificial electron acceptor (Hill reagent), replacing NADP+ to observe electron transport during photosynthesis.

The Dichloroindophenol test is a colorimetric method used to establish vitamin C concentration by measuring the reduction of DCPIP from blue to colorless.

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