Leuco Crystal Violet (LCV) is the reduced, colorless form of Crystal Violet and is a triarylmethane dye. It is a benzenoid aromatic compound that is widely used in forensic science, analytical chemistry, and biological research. LCV is oxidized to a characteristic purple-violet color in the presence of hydrogen peroxide and hemoglobin, thus making it a useful reagent for the detection of latent bloodstains on a large variety of surfaces.
In addition to forensic applications, it is employed as a chromogenic reagent in spectrophotometric assays, environmental analysis, and microbiological research. Its ability to react with proteinaceous and cellulosic materials and its reliable color-forming reaction make it a useful reagent for laboratory investigation and analytical procedures.
CAS No.: 603-48-5
Synonyms: Leucogentian violet, Leucomethyl green
| Physical Properties | |
| Chemical formula | C25H31N3 |
| IUPAC Name | 4-[bis[4-(dimethylamino)phenyl]methyl]-N,N-dimethylaniline |
| Molecular weight | 373.5 g/mol |
| Solubility | Chloroform (Slightly), Methanol (Slightly) |
| Appearance | White to Dark green powder to crystal |
| Storage | Store at Room Temperature |
| Chemical Properties | |
| Color | White to light gray to slightly lavender |
| Physical state | Solid |
| Melting Point | 171 to 181 °C |
| Boiling Point | 492.75 °C |
| Vapor Pressure | 0-0Pa at 10-30℃ |
| λmax | 260 nm |
| LogP | 5.9 |
| Odor | Odorless |
| Pictograms : |  |
| Hazard Statements : | H320 : Causes eye irritation. |
| Precautionary statements : | P264 : Wash skin thoroughly after handling. |
Leuco Crystal Violet (LCV) is the reduced, colorless form of Crystal Violet and is a triarylmethane dye. It is a benzenoid aromatic compound that is widely used in forensic science, analytical chemistry, and biological research. LCV is oxidized to a characteristic purple-violet color in the presence of hydrogen peroxide and hemoglobin, thus making it a useful reagent for the detection of latent bloodstains on a large variety of surfaces.
In addition to forensic applications, it is employed as a chromogenic reagent in spectrophotometric assays, environmental analysis, and microbiological research. Its ability to react with proteinaceous and cellulosic materials and its reliable color-forming reaction make it a useful reagent for laboratory investigation and analytical procedures.
CAS No.: 603-48-5
Synonyms: Leucogentian violet, Leucomethyl green
| Physical Properties | |
| Chemical formula | C25H31N3 |
| IUPAC Name | 4-[bis[4-(dimethylamino)phenyl]methyl]-N,N-dimethylaniline |
| Molecular weight | 373.5 g/mol |
| Solubility | Chloroform (Slightly), Methanol (Slightly) |
| Appearance | White to Dark green powder to crystal |
| Storage | Store at Room Temperature |
| Chemical Properties | |
| Color | White to light gray to slightly lavender |
| Physical state | Solid |
| Melting Point | 171 to 181 °C |
| Boiling Point | 492.75 °C |
| Vapor Pressure | 0-0Pa at 10-30℃ |
| λmax | 260 nm |
| LogP | 5.9 |
| Odor | Odorless |
| Pictograms : | |
| Hazard Statements : | H320 : Causes eye irritation. |
| Precautionary statements : | P264 : Wash skin thoroughly after handling. |
Leuco Crystal Violet is mainly used in forensic blood detection, blood impression enhancement, spectrophotometric applications, microbiological studies, and laboratory analytical applications.
Leuco Crystal Violet is reacted with hemoglobin in the presence of hydrogen peroxide to give a purple violet color, which allows the detection and documentation of blood residues on both porous and non-porous surfaces, making it useful in a variety of forensic investigations.
Leuco Crystal Violet (LCV) is distinct from Crystal Violet. LCV is the reduced, colorless form of Crystal Violet that changes into the purple-colored Crystal Violet after oxidation. This conversion makes LCV useful for forensic and analytical applications for which a visible color reaction is needed.
Leuco Crystal Violet is mainly used for laboratory and forensic applications. It should be handled in accordance with the standard laboratory safety practices, and users should refer to the product’s safety information for hazard and handling information.
Leuco Crystal Violet generates a visible purple-violet color when it reacts with hemoglobin in blood in the presence of hydrogen peroxide. It can also be oxidized with appropriate chemical oxidizing agents in analytical procedures.
