Amido Black 10B is a protein-staining azo dye widely used in biochemistry, forensic science, and histology. It works by binding to proteins and producing a distinct blue-black color, making it a reliable and effective choice for protein detection and visualization across a variety of laboratory and investigative applications. Its ability to dissolve in both methanol and water makes it a versatile reagent, suitable for a wide range of staining procedures, including Western blotting, forensic fingerprint enhancement, and van Gieson histological staining.
CAS No.: 1064-48-8
Synonyms: Acid Black 1; Amido Black 10B; Buffalo Black NBR,Naphthol Blue Black 10B, Amidoschwarz
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| Physical Properties | |
| Chemical formula | C22H14N6Na2O9S2 |
| IUPAC Name | disodium;4-amino-5-hydroxy-3-[(4-nitrophenyl)diazenyl]-6-phenyldiazenylnaphthalene-2,7-disulfonate |
| Molecular weight | 616.5 g/mol |
| Solubility | Slightly soluble in ethyl ether, benzene, DMSO, and soluble in ethanol |
| Flash Point | 30 °C |
| Density | 1.05 g/mL at 20 °C |
| Chemical Properties | |
| Color | Black to Dark Blue |
| State | Powder to Crystalline powder |
| Melting Point | >350 °C |
| Ph | 9.0 (10g/l, H2O, 25 ℃) |
| λmax | ~618 nm |
| Pictograms : |
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| Hazard Statements : | H315 – Causes skin irritation H319 – Causes serious eye irritation H335 – May cause respiratory irritation |
| Precautionary statements : | P261 – Avoid breathing dust/fume/gas/mist/vapors/spray P280 – Wear protective gloves/protective clothing/eye protection/face protection P302 + P352 – IF ON SKIN: Wash with plenty of soap and water P305 + P351 + P338 – IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing |
Amido Black 10B is a protein-staining azo dye widely used in biochemistry, forensic science, and histology. It works by binding to proteins and producing a distinct blue-black color, making it a reliable and effective choice for protein detection and visualization across a variety of laboratory and investigative applications. Its ability to dissolve in both methanol and water makes it a versatile reagent, suitable for a wide range of staining procedures, including Western blotting, forensic fingerprint enhancement, and van Gieson histological staining.
CAS No.: 1064-48-8
Synonyms: Acid Black 1; Amido Black 10B; Buffalo Black NBR,Naphthol Blue Black 10B, Amidoschwarz
Â
| Physical Properties | |
| Chemical formula | C22H14N6Na2O9S2 |
| IUPAC Name | disodium;4-amino-5-hydroxy-3-[(4-nitrophenyl)diazenyl]-6-phenyldiazenylnaphthalene-2,7-disulfonate |
| Molecular weight | 616.5 g/mol |
| Solubility | Slightly soluble in ethyl ether, benzene, DMSO, and soluble in ethanol |
| Flash Point | 30 °C |
| Density | 1.05 g/mL at 20 °C |
| Chemical Properties | |
| Color | Black to Dark Blue |
| State | Powder to Crystalline powder |
| Melting Point | >350 °C |
| Ph | 9.0 (10g/l, H2O, 25 ℃) |
| λmax | ~618 nm |
| Pictograms : |
|
| Hazard Statements : | H315 – Causes skin irritation H319 – Causes serious eye irritation H335 – May cause respiratory irritation |
| Precautionary statements : | P261 – Avoid breathing dust/fume/gas/mist/vapors/spray P280 – Wear protective gloves/protective clothing/eye protection/face protection P302 + P352 – IF ON SKIN: Wash with plenty of soap and water P305 + P351 + P338 – IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing |
Amido Black 10B is a primary protein stain for biological samples, membranes and blood-containing samples. It is widely employed in protein staining and forensics.
Amido black stain is used to give a dark blue-black color when it reacts with proteins and biological substances.
Amido Black can be applied to a variety of blood-contaminated porous and non-porous surfaces, including paper, cardboard, walls, tiles, fabrics, and other materials commonly examined during forensic investigations.
Amido black staining is based on the binding of the stain to protein in tissues, membranes and blood remains.
Amido Black is primarily used as a dye for protein staining in forensic and laboratory applications. Under normal usage conditions, it is not known to cause significant DNA damage or mutations and is not widely used for DNA visualization.Â
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Amido black 10B is indeed a frequently-used protein stain in western blotting, electrophoresis, histology and biochemical studies.
