Biebrich Scarlet is a synthetic sulfonated azo dye commonly used in histology and biological staining. It is widely employed in trichrome staining techniques to differentiate cellular and connective tissue components by imparting a red color to cytoplasmic structures.
CAS No.: 4196-99-0
Synonyms: Ponceau BS; Croceine scarlet; Fenazo Scarlet B
| Physical Properties | |
| Chemical formula | C22H14N4Na2O7S2 |
| IUPAC Name | sodium 6-(2-hydroxynaphthylazo) -3,4′-azodibenzenesulphonate |
| Molecular weight | 556.49 g/mol |
| Solubility | Soluble in Etanol and water |
| Chemical Class | Azo dye |
| Chemical Properties | |
| Colour | Dark brown red |
| State | Solid Powder |
| Odor | Odorless |
| Melting Point | 181-188 °C |
| Storage Temp. | Room Temp. |
| Pictograms : |  |
| Hazard Statements : | H315: Causes skin irritation |
| Precautionary statements : | P260: Do not breathe dust/fume/gas/mist/vapours P264: Wash thoroughly after handling P280: Wear: protective gloves/ clothing & eye protection P302+352: IF ON SKIN: Wash with soap and water P305+351+338: IF IN EYES: Rinse with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing |
Biebrich Scarlet is a synthetic sulfonated azo dye commonly used in histology and biological staining. It is widely employed in trichrome staining techniques to differentiate cellular and connective tissue components by imparting a red color to cytoplasmic structures.
CAS No.: 4196-99-0
Synonyms: Ponceau BS; Croceine scarlet; Fenazo Scarlet B
| Physical Properties | |
| Chemical formula | C22H14N4Na2O7S2 |
| IUPAC Name | sodium 6-(2-hydroxynaphthylazo) -3,4′-azodibenzenesulphonate |
| Molecular weight | 556.49 g/mol |
| Solubility | Soluble in Etanol and water |
| Chemical Class | Azo dye |
| Chemical Properties | |
| Colour | Dark brown red |
| State | Solid Powder |
| Odor | Odorless |
| Melting Point | 181-188 °C |
| Storage Temp. | Room Temp. |
| Pictograms : | |
| Hazard Statements : | H315: Causes skin irritation |
| Precautionary statements : | P260: Do not breathe dust/fume/gas/mist/vapours P264: Wash thoroughly after handling P280: Wear: protective gloves/ clothing & eye protection P302+352: IF ON SKIN: Wash with soap and water P305+351+338: IF IN EYES: Rinse with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing |
Biebrich scarlet and acid fuchsin are used together as acid dyes in histological staining, mainly to stain cytoplasm, muscle fibers, and other protein-rich tissues red. They are commonly used in trichrome staining methods, where they help differentiate these red-stained components from collagen (stained blue or green) and nuclei (stained dark), improving tissue contrast and visualization under a microscope.
Biebrich scarlet red is a synthetic azo dye belonging to the sulfonated dye class. It appears as a bright red powder and is commonly used in biological staining procedures. It functions as a biological stain and dye intermediate, particularly in histology for differentiating tissue components.
Biebrich scarlet is an acidic dye that primarily stains cytoplasmic components, connective tissues, and muscle fibers. In histological staining protocols (such as trichrome stains), it is often used to color cytoplasm and muscle tissue red, helping distinguish them from collagen and other structures.
Biebrich scarlet is an organic sodium salt used mainly as a biological stain in histology to highlight specific tissue components under a microscope.
