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RHODAMINE 6G

MacsChem > Biological Stains > RHODAMINE 6G

What is Rhodamine 6G?

Rhodamine 6G is a highly fluorescent dye from the rhodamine family, known for its bright fluorescence. It generally comes in 3 forms: chloride (C28H31ClN2O3), perchlorate (C28H31ClN2O7), and tetrafluoroborate (C28H31BF4N2O3), each with distinct solubility and properties. It has an excitation maximum of around 530 nm and an emission maximum of approximately 550-610 nm, with strong fluorescence in the yellow-orange region.

CAS No.: 989-38-8

Synonyms: Basic Red 1; Rhodamine F5G; Rhodamine 6G chloride; Calcozine Rhodamine 6GX; Rhodamine 6G extra; Aizen Rhodamine 6GCP; Rhodamine 6G hydrochloride; Rhodamine 6G, indicator; Rhodanine 6GDN; Rhodamine 6GO

Properties of Rhodamine 6G

Physical Properties
Chemical formulaC28H31ClN2O3
IUPAC NameHydrogen ethyl2-[6-(ethylamino)-3-(ethylimino)-2,7-dimethyl-3H- xanthen-9-yl]benzoate chloride
Molecular weight479.02 g/mol
SolubilityWater & Ethanol
Flash point318.6°C
Density1.15 g/cm³
Chemical Properties
ColorMaroon, red, red-brown, bright bluish-pink, and reddish purple
StateSolid Powder or Crystals
Melting point290°C
λmax 565 nm
pH6.0
Log P7.22550
Absorbance530 nm

Uses of Rhodamine 6G

  • Tracer Dye: Rhodamine 6 G is often used to trace water flow and movement due to its intense fluorescence, which makes diagnosis easy with fluorometers.
  • Dye Laser: Rhodamine 6G is employed as a laser dye in dye lasers, especially for generating light in the 570-660 nm range, due to its high photostability, efficiency, and high quantum yield.
  • Fluorescence: Rhodamine 6G is extensively used in biotechnology, such as fluorescence microscopy, flow cytometry, and fluorescence correlation spectroscopy
  • ELISA Assays: Rhodamine 6G dye is also used in Enzyme-Linked Immunosorbent Assays (ELISA) for detecting precise proteins or antibodies.
  • Raman Spectrum: The Raman spectrum of Rhodamine 6G shows distinct peaks between 600 to 1700 cm⁻¹, highlighting C-C stretching and C-H bending vibrations. Due to strong fluorescence, techniques like SERS are often used to enhance the Raman signal.
  •  
Pictograms : 
Hazard Statements :

H30: Toxic if swallowed.
H317: This may cause an allergic skin reaction.
H318: Causes serious eye damage.
H410: Very toxic to aquatic life with long-lasting effects.

Precautionary statements :

P261: Avoid breathing dust/ fume/ gas/ mist/ vapors/ spray.
P273: Avoid release to the environment.
P280: Wear protective gloves/ eye protection/ face protection.
P301 + P310: IF SWALLOWED: Immediately call a POISON CENTER/ doctor.
P302 + P352: IF ON SKIN: Wash with plenty of water.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present, and easy to do. Continue rinsing.

What is Rhodamine 6G?

Rhodamine 6G is a highly fluorescent dye from the rhodamine family, known for its bright fluorescence. It generally comes in 3 forms: chloride (C28H31ClN2O3), perchlorate (C28H31ClN2O7), and tetrafluoroborate (C28H31BF4N2O3), each with distinct solubility and properties. It has an excitation maximum of around 530 nm and an emission maximum of approximately 550-610 nm, with strong fluorescence in the yellow-orange region.

CAS No.: 989-38-8

Synonyms: Basic Red 1; Rhodamine F5G; Rhodamine 6G chloride; Calcozine Rhodamine 6GX; Rhodamine 6G extra; Aizen Rhodamine 6GCP; Rhodamine 6G hydrochloride; Rhodamine 6G, indicator; Rhodanine 6GDN; Rhodamine 6GO

Properties of Rhodamine 6G

Physical Properties
Chemical formulaC28H31ClN2O3
IUPAC NameHydrogen ethyl2-[6-(ethylamino)-3-(ethylimino)-2,7-dimethyl-3H- xanthen-9-yl]benzoate chloride
Molecular weight479.02 g/mol
SolubilityWater & Ethanol
Flash point318.6°C
Density1.15 g/cm³
Chemical Properties
ColorMaroon, red, red-brown, bright bluish-pink, and reddish purple
StateSolid Powder or Crystals
Melting point290°C
λmax 565 nm
pH6.0
Log P7.22550
Absorbance530 nm

Uses of Rhodamine 6G

  • Tracer Dye: Rhodamine 6 G is often used to trace water flow and movement due to its intense fluorescence, which makes diagnosis easy with fluorometers.
  • Dye Laser: Rhodamine 6G is employed as a laser dye in dye lasers, especially for generating light in the 570-660 nm range, due to its high photostability, efficiency, and high quantum yield.
  • Fluorescence: Rhodamine 6G is extensively used in biotechnology, such as fluorescence microscopy, flow cytometry, and fluorescence correlation spectroscopy
  • ELISA Assays: Rhodamine 6G dye is also used in Enzyme-Linked Immunosorbent Assays (ELISA) for detecting precise proteins or antibodies.
  • Raman Spectrum: The Raman spectrum of Rhodamine 6G shows distinct peaks between 600 to 1700 cm⁻¹, highlighting C-C stretching and C-H bending vibrations. Due to strong fluorescence, techniques like SERS are often used to enhance the Raman signal.
  •  
Pictograms : 
Hazard Statements :

H30: Toxic if swallowed.
H317: This may cause an allergic skin reaction.
H318: Causes serious eye damage.
H410: Very toxic to aquatic life with long-lasting effects.

Precautionary statements :

P261: Avoid breathing dust/ fume/ gas/ mist/ vapors/ spray.
P273: Avoid release to the environment.
P280: Wear protective gloves/ eye protection/ face protection.
P301 + P310: IF SWALLOWED: Immediately call a POISON CENTER/ doctor.
P302 + P352: IF ON SKIN: Wash with plenty of water.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present, and easy to do. Continue rinsing.

FAQ's

It is primarily used as a laser dye and fluorescent tracer and in several biotechnology applications such as flow cytometry, and ELISA. 

Yes, Rhodamine 6G, especially in its chloride form, is corrosive to most metals except stainless steel.

Rhodamine 6G dye is cationic.

Rhodamine 6G has both hydrophilic and hydrophobic properties, but it is generally more hydrophilic due to its solubility in water. However, it does have some hydrophobic characteristics, which can allow it to interact with non-polar environments.

 The Rhodamine 6G test detects leaks or flows in water systems using its fluorescent properties.

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